TWC BioSearch
Discount Research Software Agent
Advanced Interactive Gas Chromatography
This recently released training package, written by a experienced chromatographer,
Geoff Pickerell of Chromaster, contains a wealth of practical information
about the use of packed and capillary columns, the principals of integration,
and the "ways and means"' of method development, with self-test questions
on each section.
The five modules included are as follows:
GC Requirements
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Introduction: objectives, sources of methods.
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Revision of Principles: gas chromatography, gas-solid chromatography, gas-liquid
chromatography.
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Sample Handling:
| Sample Requirements: gases, liquid samples, solid samples |
| Sample Clean Up: solvent extraction, soxhlet extraction,
solid phase extraction |
| Sample Derivatization: improved volatility and separation,
improved sensitivity |
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Instrument Requirements: packed and capillary columns: pros and cons, column
handling
GC Separation
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Column Selection: stationary phase selection: packed and capillary columns,
column selectivity, comparison of stationary phases
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Temperature Program Facility: introduction, initial temperature, program
rate, initial isothermal period
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GC: Separation Strategy
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Operation of packed columns: introduction, initial conditions, optimization:
packed column analysis, temperature adjustment, temperature optimization,
temperature program
Capillary Operation
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Operation of Capillary Columns: stationary phase selection, column geometry,
initial conditions, carrier gas and flow rate, choice of gas
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Capillary Optimization: residence time, linear velocity, stationary phase,
temperature program
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Capillary Injection: split injection, split ratio, injection technique,
hot needle technique, splitless injection, opening split value, non-vaporizing
injection, on-column injection - with example, programmed temperature injector,
programmed split mode, programmed splitless mode, solvent purge mode, retention
gaps
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Further Capillary Concepts: optimum practical gas velocity, phase ratio
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Megabore Columns: installation and operation
Qualitative and Quantitative Analysis
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Principles of Integration: sampling rate, peak width changes, bunch rate,
data collection, peak detection - with demonstration, slope threshold,
effect of minimum area value
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Integration of Fused Peaks: demonstrations, baseline codes, timed events
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Method validation:
| Selectivity, confirmation of peak identity, spiking, Kovacs
index |
| System suitability tests, inlet degradation, column degradation,
separation number |
| Method ruggedness |
| Accuracy, precision, linearity |
| Minimum detectable quantity, minimum quantifiable level |
Questions and Answers
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There are ten questions on each of the four modules
Interactive GC Applications and Method Development is one of a range of
training packages for analytical chemistry. Also available are Interactive
GC, HPLC, AAS, ICP and MS, each giving a basis and through understanding
of, and training in the use of, each technique. They are suitable for both
university students and laboratory staff, giving sufficient emphasis to
both theory and practice. Network versions are available.
There is now a wealth of research demonstrating the advantages of computer-based
training as compared to traditional methods: increased understanding and
retention, shorter training time, constant availability, flexibility of
uses and considerable cost savings. Training is no longer dependent upon
having access to instruments, and can be tailored to individual requirements.
Trainees can watch the animation demonstrating the working of the instrument;
adjust parameters to see the effect; pursue their own lines of inquiry;
and return to areas of uncertainty. The software has a free flow, open-ended
structure which enables the user to browse freely through its pages, or
to follow the programmed order. A useful session may last 10 minutes or
several hours.
Requires Windows 3.1, 95, 98 or NT
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