TWC BioSearch
Signal Transduction Supplier

GENE REGULATION

VITAMIN D RECEPTOR

DM-200
1,25-Dihydroxyvitamin D3 (Calcitriol)
99%, MW=416.6 [32222-06-3] Storage: -70°C
1,25-Dihydroxyvitamin D3 is the active hormonal form of vitamin D1. It is a central regulator of bone and calcium homeostasis. Polymorphism in the vitamin D receptor gene has recently been linked to a genetic disposition to osteoporosis2,3. It inhibits proliferation and induces differentiation of a variety of cells including osteoclasts4, keratinocytes5, HL-60 cells6 vascular smooth muscle cells7 and bone marrow macrophage precursors8. It can activate signal transduction pathways that include hydrolysis of phosphatidylcholine and formation of diacylglycerol in chick myoblasts9, and activation of Raf kinase in rat hepatic primary cells and 3T3 fibroblasts10. It regulates c-myc protooncogene transcription11, interleukin-8 expression12 and induction of cyclooxygenase13. It directly activates protein kinase C at physiological concentrations (EC50=16 nM)14.
50 µg
5 x 50 µg
1 mg

DM-100
25-Hydroxyvitamin D3
99%, MW=400.6 [19356-17-3] Storage: -20°C
Intermediate in the biosynthesis of 1,25-dihydroxyvitamin D3. Major circulating metabolite of vitamin D315.
1 mg
5 mg

DM-300
24(R), 25-Dihydroxyvitamin D3
99%, MW=416.6 [40013-87-4] Storage: -70°C
Major metabolite of vitamin D31. Mediates calcium and phosphorous homeostasis16. Inhibits 1,25-dihydroxyvitamin D3 and testosterone potentiation of calcium channels in osteosarcoma cells17.
50 µg
5 x 50 µg

SE-140
Vitamin D3 Receptor (human, recombinant)
>75% by SDS-PAGE, 48 kDa, Storage: -20°C
Purified vitamin D3 receptor expressed in a baculovirus expression system. This product retains native vitamin D3 affinity and ability to bind the human osteocalcin gene vitamin D response element (VDRE). Supplied at ~4 µM in 50% glycerol buffer. Specific activity: Binds 4.9 nmol 1a,25-dihydroxyvitamin D3/mg protein. Applications: To study receptor/ligand binding; transcriptional activation of VDRE's; quantification of 1a,25-dihydroxyvitamin D3 in samples18,19,20.
750 pmol

SA-180
Anti-Vitamin D receptor (VDR)
Rat monoclonal antibody. Clone 9A7. Immunogen: Partially purified chicken intestinal cytoplasmic 1,25-dihydroxyvitamin D3 receptor protein21,22. Supplied as purified IgG2b, 1 mg/ml. Recognizes VDR epitope C-terminal to the DNA binding zinc finger domain. Reacts with occupied and unoccupied receptor. Cross-reacts with all avian and mammalian VDR. No cross-reaction with glucocorticoid and estrogen receptors, or serum and cytosolic vitamin D-binding proteins23,24,25. Applications: WB 1 µg/ml, IC 5 µg/ml, IP.
50 µg
NEW LOWER PRICE

RETINOIDS

GR-104
AM-580
98%, MW=351.4 [102121-60-8] Storage: -20°C
Retinoic acid analog which acts as a selective RARa agonist26 with no affinity for RXR's27. Induces differentiation in leukemia cell lines28,29, and inhibits angiogenesis in chick embryos30.
5 mg
25 mg

GR-103
4-Hydroxyphenylretinamide
97%, MW=391.5 [65646-68-6] Storage: -20°C
An analog of all trans retinoic acid that shows antiproliferative activity on cultured human breast cancer cells31,32. Highly selective activator of retinoid receptors33. Induces apoptosis in malignant hemopoietic cell lines34,35.
5 mg
25 mg

GR-106
Methoprene acid
11-Methoxy-3,7,11-trimethyl-2E,4E-dodecadienoic acid
98%, MW=268.4, Storage: -20°C
Methoprene acid is an analog of the insect growth regulator, juvenile hormone. It directly binds to RXR and is a transcriptional activator in both insect and mammalian cells. It is RXR specific and does not activate the RAR pathway36.
5 mg
25 mg

GR-100
Retinoic acid, all trans
>95%, MW=300.4 [302-79-4] Storage: -20°C
Retinoic acid is the active metabolite of vitamin A. It is a potent modulator of growth and differentiation37 exerting its effects by binding to nuclear retinoic acid receptors (RARs) which directly regulate gene expression38. All-trans retinoic acid binds to RAR , and subtypes with Kd values of 0.2, 0.4 and 0.2 nM respectively39. RARs function as ligand-dependent transcription factors38,40.
500 mg
5 g

GR-101
9-cis Retinoic acid
99%, MW=300.4 [5300-03-8] Storage: -20°C
A high affinity ligand for the retinoid X receptor (RXR)41,42,43. Destabilizes VDR-RXR heterodimer-DNA complex44. Biosynthesis via isomerization of all trans retinoic acid45.
5 mg
25 mg

GR-102
13-cis Retinoic acid
>95%, MW=300.4 [4759-48-2] Storage: -20°C
A potent modulator of growth and differentiation46. Clinically used in a variety of skin disorders47,48,49.
100 mg
500 mg

GR-105
TTNPB (Ro 13-7410)
4-[E-2-(5,6,7,8-Tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)-1-propenyl]benzoic acid
98%, MW=348.5 [71441-28-6] Storage: -20°C
Retinoic acid analog which acts as a selective RAR agonist50,51.
5 mg
25 mg

SA-157
Anti-Retinoic acid receptor (RAR)
Rabbit polyclonal antibody. Immunogen: Mouse RAR (444-462) C-terminal peptide52. Supplied as serum. Recognizes human and mouse ~45 kDa RAR. No cross-reactivity with RAR or isotypes. Applications: WB 1/1000.
100 µl

SA-178
Anti-Retinoic acid receptor (RAR)
Mouse monoclonal antibody. Clone R10. Immunogen: Mouse RAR (444-462) C-terminal peptide52. Supplied as IgG1, lyophilized. Recognizes human, rat and mouse RAR in cell lines and tissue extracts. No cross-reactivity with RAR or isotypes. Applications: WB 1-2 µg/ml.
50 µg

SA-158
Anti-Retinoic acid receptor (RAR)
Rabbit polyclonal antibody. Immunogen: Mouse RAR (429-448) C-terminal peptide52. Supplied as serum. Recognizes human and mouse RAR. Slight cross-reactivity to RAR but none to . Applications: WB 1/1000.
100 µl

SA-179
Anti-Retinoic acid receptor (RAR)
Mouse monoclonal antibody. Clone R28. Immunogen: Mouse RAR (429-448) C-terminal peptide52. Supplied as IgG2a, lyophilized. Recognizes human and mouse but not rat RAR. No cross-reactivity with RAR or in cell lines or tissue extracts. WB 1-2 µg/ml.
50 µg

SA-203
Anti-Retinoic X receptor b (RXRb)
Mouse monoclonal antibody. Clone MOK13-17. Immunogen: Recombinant human RXRb. Supplied as 2 mg/ml IgG1 in PBS diluted ascites. Recognizes human and mouse RXRb ~50 kDa. No cross-reactivity with RXRa or g isotypes. Applications: WB 5 µg/ml, IP and IF. Gel super-shift assays show disruption of the VDR/RXRb/VDRE (DNA) complex when RXRb was preincubated with MOK13-17. In contrast, THR/RXRb heterodimer show a supershift by this antibody53-56.
25 µg
100 µg

SE-127
Retinoid X receptor b (RXRb) (human, recombinant)
Partially purified, 51 kDa, Storage: -70°C
Entire coding region of RXRb (rH2RIIBP) expressed in baculovirus/Sf953-56. Supplied partially purified at 2 mg/ml total protein (~0.6 mg/ml RXRb). Applications: Gel shift assays (3 µg); Western blotting control for SA-203, above.
50 µg
5 x 50 µg

PEROXISOME PROLIFERATOR-ACTIVATED RECEPTORS (PPARS)

Peroxisome proliferators are a diverse group of substances which induce a massive accumulation of peroxisomes in hepatocytes and strongly induce enzymes of the peroxisomal and microsomal fatty-acid oxidation systems. Peroxisome proliferator-activated receptors, PPARs, belong to a family of nuclear hormone receptors shared by thyroid, retinoid and vitamin D receptors. PPARs can heterodimerize with retinoid X receptor (RXR) and directly regulate gene transcription via interaction with response elements57,58,59.

GR-205
Ciglitizone
MW=333.5 [74772-77-3]
Ciglitizone is a thiazolidinedione type of antihyperglycemic agent. It displays antihyperglycemic activity in genetically obese C57 B1/6 ob/ob mice60 and is a selective PPARg agonist (EC50=3 mM)61.
5 mg
25 mg

EI-160
Clofibrate
2-(p-Chlorophenoxy)-2-methylpropionic acid, ethyl ester
Peroxisome proliferator that induces cytochrome P-450 IVA1 and IVA3.

ET-004
5,8,11,14-Eicosatetraynoic acid (ETYA)
ETYA is 100-fold more effective than WY-14643 at activation of PPAR.

RA-101
LY 171883
Activates peroxisome proliferator-activated receptors (PPARs)62.

PG-050
15d-PGJ2 (15-deoxy-D12,14-Prostaglandin J2)
15d-PGJ2 is a metabolite of PGD2 recently shown to activate PPARg-dependent adipogenesis in transfected 3T3 cells, and activates a PPARg response element in CV-1 cells. 15d-PGJ2 competes with thiazolidinediones for PPARg binding. It has been proposed to be a natural ligand for PPARg63,64.

GR-200
WY-14643
4-Chloro-6-(2,3-xylidino)-2-pyrimidinylthioacetic acid
98%, MW=323.8 [50892-23-4] Storage: RT
WY-14643 is a potent and widely used PPAR activator65,66,67. It has been shown to be selective for PPARa68.
50 mg
250 mg

SA-206
Anti-PPARg1,2, Peroxisome proliferator-activated receptors
Rabbit polyclonal antibody. Immunogen: Mouse PPARg2 peptide (284-298), identical to PPARg1 conjugated to a carrier69,70,71. Supplied as serum. Recognizes human, rat and mouse PPARg1,2. No crossreactivity to PPARa or NUCI. Applications: WB 1/2000 and gel super-shift assays.
100 µl

SA-207
Anti-PPARs, Peroxisome proliferator-activated receptors
Rabbit polyclonal antibody. Immunogen: Mouse PPARg2 C-term peptide (484-498) conjugated to a carrier. This sequence is highly conserved in human, rat and mouse PPARg1, PPARa and PPARd (NUCI) 69,70,71. Supplied as serum. Recognizes rat PPARa, mouse PPARg1 and PPARg2 and others. Applications: WB 1/2000 and gel super-shift assays (PPARg2 in mouse 3T3L1).
100 µl


LEPTIN

Obese (OB) Gene Product

Insight into the genetic regulation of obesity has come from the study of the ob/ob mouse. These mice grow massively obese and develop diabetes mellitus due to overeating and increased metabolic efficiency. Recently the obese gene was cloned and shown to encode the leptin protein. Mature, secreted leptin is 146 amino acids in length and is a unique protein. The obese mRNA is expressed selectively in adipose tissue. Treatment of ob/ob mice with leptin caused reversal of the obese phenotype72-75.

SE-161
Leptin (human, recombinant )
>95% by SDS-PAGE, MW=16 kDa, Storage: -20°C
Leptin (obese gene product) is a ligand for the OB receptor. This 146 amino acid recombinant protein is expressed in E. coli. It is highly purified and biologically active. Supplied lyophilized.
0.5 mg
5 x 0.5 mg

SE-160
Leptin (murine, recombinant )
>95% by SDS-PAGE, MW=16 kDa, Storage: -20°C
Same as SE-161 above, but this is mouse species. Supplied lyophilized.
1 mg
5 x 1 mg

SA-268
Anti-Leptin, Human and Mouse
Rabbit polyclonal antibody. Immunogen: Human and mouse recombinant leptins (obese gene product)76. Supplied as purified IgG, 1 mg/ml. Recognizes 16 kDa human and mouse 16 kDa leptin. Applications: WB 1 µg/ml (using alkaline phosphatase and BCIP/NBT), ELISA.
100 µg

SA-215
Anti-Leptin (OB gene product)
Rabbit polyclonal antibody. Immunogen: Mouse leptin (obese gene product) peptide residues 25-44 conjugated to a carrier. Supplied as peptide antigen immunoaffinity purified IgG. Recognizes mouse recombinant 16 kDa leptin and leptin in mouse 3T3-L1 cells induced to differentiate into adipocytes. Crossreacts with recombinant human leptin in WB. Applications: WB 1/2000, ELISA for mouse.
50 µl

NF-kB

NF-kB INHIBITORS

NF-kB is a transcriptional activator of many pro-inflammatory proteins. The NF-kB inhibitor protein IkB is degraded by the proteasome, thereby releasing activated NF-kB. Several proteasome inhibitors such as MG-132 and lactacystin can penetrate cells and inhibit NF-kB activation.

PI-102
MG-132
Z-Leu-Leu-Leu-CHO
A potent and selective reversible proteasome inhibitor (Ki= 4 nM). Inhibits NF-kB activation (IC50=3 mM)77.

PI-104
Lactacystin
A potent and selective irreversible proteasome inhibitor78. Inhibits NF-kB activation (IC50=10 mM)3. Induces neurite outgrowth in Neuro 2A mouse neuroblastoma cells79.

P-600
NF-kB SN50, cell-permeable inhibitory peptide
>97%, MW=2782, Storage: -20°C
Sequence contains the nuclear localization sequence (NLS residues 360-369) of the transcription factor NF-kB p50 linked to a peptide cell-permeablization sequence, the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF). Peptide inhibits translocation of the NF-kB active complex into the nucleus. In murine endothelial LE-II cells induced with LPS, inhibition of NF-kB nuclear translocation was 88% at 50 µg/ml (18 µM). In human monocytic THP-1 cells induced by LPS or TNFa there was 85% inhibition at 100 µg/ml80.
0.5 mg
5 x 0.5 mg

P-601
NF-kB SN50M, cell-permeable control peptide
>97%, MW=2668, Storage: -20°C
Inactive control peptide for the cell-permeable NF-kB inhibitory peptide SN50 (P-600, above). Contains the nuclear localization sequence (NLS residues 360-369) of the transcription factor NF-kB p50 linked to the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF), except this control peptide has Lys-363 to Asn and Arg-364 to Gly. In murine endothelial LE-II cells induced with LPS, SN50M peptide had no measureable effect on NF-kB translocation at 50 µg/ml. In human monocytic THP-1 cells induced by LPS or TNFa, there was slight inhibition of nuclear transport at 100 µg/ml80.
0.5 mg
5 x 0.5 mg

PG-001
Prostaglandin A1
Prostaglandin A1 is a potent inhibitor of NF-kB activation in human cells (3-48 mM). It acts by inhibiting phosphorylation and preventing degradation of IkB81.

NF-kB ANTIBODIES

SA-170
Anti-NF-kB p50 subunit
Rabbit polyclonal antibody. Immunogen: Human NF-kB p50 N-terminal peptide conjugated to KLH. Supplied as serum. Reacts with human but not mouse p50 and precursor protein p105. Applications: WB 1/1000, IP, ELISA, Gel supershift assay (reactive against p50:p50 homodimers using 1 µl per assay; less reactive for p50:p65 heterodimers). Supplied with 50 µg of control peptide.
100 µl

SA-171
Anti-NF-kB p65 subunit
Rabbit polyclonal antibody. Immunogen: Human NF-B p65 C-terminal peptide conjugated to KLH. Supplied as serum. Reacts with human and mouse p65. Applications: WB 1/1000, IP, ELISA, Gel supershift assay (reactive against all human and mouse p65 containing NF-B complexes using 1 µl per assay). Supplied with 50 µg of control peptide.
100 µl

SA-172
Anti-NF-kB c-rel
Rabbit polyclonal antibody. Immunogen: Human c-rel C-terminal peptide conjugated to KLH. Supplied as serum. Recognizes human but not mouse c-rel. Applications: WB 1/1000, IP, ELISA, Gel supershift assay (reactive against NF-kB complexes containing c-rel homo- and heterodimers using 1 µl per assay). Includes 50 µg of control peptide.
100 µl

SA-173
Anti-IBa, NF-kB regulatory subunit
Rabbit polyclonal antibody. Immunogen: Human IBa N-terminal peptide conjugated to KLH. Supplied as serum. Recognizes human but not mouse IBa. Applications: WB 1/1000, IP, ELISA. Includes 50 µg of control peptide.
100 µl

SA-238
Anti-IBb, NF-kB regulatory subunit
Rabbit polyclonal antibody. Immunogen: Human IBb C-terminal peptide conjugated to KLH. Supplied as serum. Recognizes human, rat and mouse IBb. Applications: WB 1/1000, IP, ELISA. Includes 50 µg of control peptide.
100 µl

SA-174
Anti-NF-kB kit, polyclonal antibodies and peptides
Contains c-rel, IBa, p50 and p65 rabbit polyclonal antibodies (SA-170 to SA-173; 100 µl each) and corresponding control peptides (50 µg each). Also includes GAR-HRP secondary antibody (2 mg) specifically tested for use with the above antibodies. The kit supplies reagents suitable for the analysis of human NF-kB transcription factor and regulator IBa. For use in WB, IP, ELISA and gel supershift assay.
1 Kit


OTHER GENE REGULATION REAGENTS

GR-300
Actinomycin D
98% MW=1255.5 [50-76-0] Storage: -20°C
A widely used intercalating transcription inhibitor.
5 mg
5 x 5 mg

GR-301
Camptothecin
97%, MW=348.4 [7689-03-4] Storage: 0°C
Reversibly inhibits nuclear topoisomerase I by binding to and stabilizing the topoisomerase-DNA covalent complex82,83,84. It inhibits Tat-mediated transactivation of HIV-185. Induces apoptosis in osteosarcoma86 and hepatoma cells87.
50 mg
250 mg

GR-302
Chromomycin A3
98% MW=1183.3 [7059-24-7] Storage: -20°C
Chromomycin A3 is a GC-specific DNA ligand which inhibits transcription88. It may be used as a fluorescent DNA stain89,90.
10 mg
5 x 10 mg

GR-310
Cycloheximide
95%, MW=281.4 [66-81-9] Storage: RT
Inhibits protein synthesis in eucaryotes by interfering with the translocation step. Induces apoptosis in a variety of cells91,92 but also can delay93 or inhibit94 apoptosis induced by other agents.
5 g

GR-307
Etoposide (VP-16)
>97%, MW=588.6 [33419-42-0] Storage: RT
Topoisomerase II inhibitor95. Widely used apoptosis inducer. Clinically useful cancer therapeutic agent96,97.
100 mg
500 mg

GR-303
HOE-33342?HCl (Bisbenzimide)
2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2',5'-bi-1H-benzimidazole
>95%, MW=561.9 [23491-52-3] Storage: -20°C
HOE-33342 is an AT-specific DNA minor groove ligand88. Induces cell differentiation90. May be used as a fluorescent DNA stain98.
100 mg
5 x 100 mg

GR-304
Homoharringtonine
98%, MW=545.6 [26833-87-4] Storage: -20°C
Homoharringtonine is a cephalotaxine alkaloid that induces heat protection and facilitates dissociation of heat shock transcription factor and heat shock element complex99. Shows antiproliferative activity in various cancer cell lines100,101.
5 mg
5 x 5 mg

GR-308
b-Lapachone
98%, MW=242.4 [4707-32-8] Storage: RT
Novel DNA topoisomerase I inhibitor. Unlike camptothecin, b-lapachone does not stabilize the cleavable complex, indicating a novel mode of action102. Induces apoptosis in HL-60 and human prostate cancer cells via a p53-independent mechanism and blocks cell cycle at G0/G1103.
5 mg
25 mg

GR-313
Lycorine HCl
98%, MW=323.8 [476-28-8] Storage: -20°C
Lycorine is a eucaryotic termination inhibitor104.
10 mg
50 mg

GR-305
Mithramycin A
>96%, MW=1085.2 [18378-89-7] Storage: -20°C
Mithramycin noncovalently interacts with G-C-rich duplex DNA in the presence of Mg2+ or Zn2+ 105. It induces differentiation of leukemic cells accompanied by an early decrease in c-myc expression106,107. It selectively inhibits collagen-1 gene expression in human fibroblasts108.
1 mg
5 x 1 mg

GR-311
Mitomycin C
95%, MW=334.3 [50-07-7] Storage: -20°C
A DNA crosslinking and damaging agent. Inhibits DNA synthesis109. Induces apoptosis in a variety of cells via a p53-dependent pathway.
2 mg
10 mg

A-256
Novobiocin sodium (U-6591)
>90%, MW=634.6 [1476-53-5] Storage: 0°C
Novobiocin inhibits topoisomerase II111, causes DNA relaxation112 and changes in DNA repair113. It prevents radiation-induced alterations in DNA114 and enhances the cytotoxicity of alkylating agents and cisplatin115.
1 g
5 g

GR-312
Puromycin?HCl
98%, MW=544.4 [3506-23-8] Storage: -20°C
Protein synthesis inhibitor. Causes premature chain termination. Induces apoptosis in a variety of cells116,117. Inhibits a "puromycin sensitive" aminopeptidase and arrests cells in G2/M phase118.
50 mg
250 mg

GR-306
Rifampicin
3-[4-Methylpiperazinyliminomethyl]rifamycin SV
>95%, MW=823.0 [13292-46-1] Storage: 0°C
A selective inhibitor of bacterial DNA-dependant RNA polymerase (RNAP). Has no effect on mammalian RNA polymerase. The RNAP-rifampicin complex locks the promoter in an abortive initiation reaction making short oligoribonucleotides which diffuse out of the active site119.
1 g
5 x 1 g

A-282
Streptolydigin (U-5481)
98%, MW=600.7 [7229-50-7] Storage: 0°C
Inhibits assembly of the RNA polymerase II transcription complex in bacteria120. Inhibits RNA polymerase III transcription121.
10 mg
50 mg

GR-309
Trichostatin A
99%, MW=302.4 [58880-19-6] Storage: -20°C
Reversible acetylation of lysines located at the conserved domain of core histones plays an important role in the regulation of chromatin structure and its transcriptional activity122,123,124. Trichostatin A (TSA) is a potent and reversible inhibitor of histone deacetylase. In HeLa cells, TSA blocked cell cycle progression at G1 and induced a 12-fold increase in intracellular levels of gelsolin125. In cells latently infected with HIV-1, TSA induced the transcriptional activation of the HIV-1 promoter, which resulted in a marked increase in virus production126. In NIH 3T3 cells, TSA induced reversion of oncogenic ras-transformed cells to a normal morphology127. In Jurkat cells, TSA inhibited IL-2 gene expression (IC50=73 nM) and displayed immunosuppressive activity in a mouse model128. Trichostatin A is a new useful tool for induction of hyperacetylation of cellular histones and for further elucidation of their role in gene expression.
1 mg
5 x 1 mg


OTHER TRANSCRIPTION FACTOR ANTIBODIES

SA-210
Anti-AhR (Aryl hydrocarbon receptor)
Rabbit polyclonal antibody. Immunogen: Recombinant N-term fragment of B-1 AhR (residues 1-402)129. Recognizes AhR ~95 kDa protein in rat and mouse liver. No cross-reactivity to Arnt.
INQUIRE

SA-138
Anti-c-myc
Mouse monoclonal antibody. Clone 9E10. Immunogen: Human pp62 c-myc (408-432) peptide130,131. Supplied as IgG1 ascites. Target is c-myc epitope EQKLISEEDL. Will detect c-myc in cells that overexpress the protein. Applications: WB 1/1000, IF, IP for epitope tagging only.
200 µl
5 x 200 µl

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