DEUTERIUM LABELED EICOSANOIDS AND LIPIDS

Gas chromatography/mass spectrometry (GCMS) is the most sensitive and specific method for measurement of eicosanoids^1-9 and PAF^10-12. Sensitivities on the picogram level are routinely achieved using GCMS with selected ion monitoring. A basic requirement for a specific and precise quantitative assay is the availability of stable isotope labeled target standards. Such standards are added to the biological sample immediately following collection and not only serve as internal standards for final quantitation but reflect the recovery of the target eicosanoid through isolation, purification and derivatization steps.

PROSTAGLANDINS

PG-044
Prostaglandin D₂-16,17,18,19-d₄
>98%, 50 µg/0.5 ml methanol, <0.2% d₀
MW=356.5, Storage: -80°C
50 µg
5 x 50 µg

PG-040
2,3-Dinor-6-keto-prostaglandin F_1a-20,20,20-d₃
>98%, 50 µg/0.5 ml ethyl acetate, 0.1-0.8% d₀,
MW=345.5, Storage: -20°C
50 µg
5 x 50 µg

PG-032
6-Keto-prostaglandin F_1a-3,3,4,4-d₄
>98%, 50 µg/0.5 ml methanol, 98 atom %D
MW=374.5, Storage: -20°C
50 µg
5 x 50 µg

TB-402
Thromboxane B₂-3,3,4,4-d₄
>98%, 50 µg/0.1 ml methanol, <0.1% d₀
MW=374.5, Storage: -20°C
50 µg
5 x 50 µg

LEUKOTRIENES

LB-504
Leukotriene B₄-6,7,14,15-d₄
>99%, 50 µg/ml ethanol, <0.2% d₀
MW=340.5, Storage: -80°C
50 µg
5 x 50 µg

HETEs

H-615
15(S)-HETE-d₈
>98%, 50 µg/ml ethanol, >98 atom %D
MW=328.5, Storage: -20°C
Labeled at the 5,6,8,9,11,12,14,15-positions.
50 µg
5 x 50 µg

EETs

EE-656
(?5,6-EET-d₈ methyl ester
>98%, 50 µg/ml ethanol, >98 atom %D
MW=342.5, Storage: -20°C
50 µg
5 x 50 µg

EE-689
(?8,9-EET-d₈
>98%, 50 µg/ml ethanol, >98 atom %D
MW=328.5, Storage: -20°C
50 µg
5 x 50 µg

EE-611
(?11,12-EET-d₈
>98%, 50 µg/ml ethanol, >98 atom %D
MW=328.5, Storage: -20°C
50 µg
5 x 50 µg

EE-614
(?14,15-EET-d₈
>98%, 50 µg/ml ethanol, >98 atom %D
MW=328.5, Storage: -20°C
50 µg
5 x 50 µg

FATTY ACIDS

FA-503
Arachidonic acid-5,6,8,9,11,12,14,15-d₈
98%, 99 atom %D
MW=312.5, Storage: -20°C
10 mg
50 mg

REFERENCES

1. G.A. FitzGerald et al. Adv. Prostagland. Thromboxane Leukotriene Res. 1985 15 87

2. J.A. Lawson et al. Anal. Biochem. 1985 150 463

3. C. Fischer et al. Biomed. Mass Spectrom. 1985 12 399

4. C.O. Meese et al. Biomed. Mass Spectrom. 1985 12 554

5. F. Fitzpatrick et al. Thromb. Res. 1984 35 121

6. J.Y. Westcott et al. Biomed. Mass Spectrom. 1985 12 714

7. J.M. Boeynaems et al. Anal. Biochem. 1980 104 259

8. C. Prakash et al. Prostaglandins 1989 37 251

9. I.A. Blair Methods Enzymol. 1990 187 13

10. K.L. Clay Methods Enzymol. 1990 187 134

11. W.C. Pickett and C.S. Ramesha Methods Enzymol. 1990 187 142

12. M. Balazy et al. Anal. Biochem. 1991 196 1