ENZYMES AND ANTIBODIES

ENZYMES

PL-005
5-Lipoxygenase (from potato)^1,2,3
98%, Supplied in 2 M ammonium sulfate containing 0.15 M potassium phosphate, pH 6.3. Specific activity: with linoleic acid is 24 units/mg protein, with arachidonic acid is 8 units/mg protein. One unit will consume one micromole of oxygen per minute at 30°C¹. Storage: -80°C.
1.0 mg

PL-012
12-Lipoxygenase (from porcine leukocytes)⁴
Semi-purified, supplied in 20 mM Tris·HCl buffer, pH 7.2, containing 0.5 mM EDTA, PMSF and DTT. Specific activity: approximately 50 units/mg protein. One unit converts one nanomole of arachidonic acid to 12-HPETE per minute at 20°C. Storage: -80°C.
100 units

PL-015
15-Lipoxygenase (from rabbit reticulocytes)^5,6,7
Semi-purified. Supplied in 10 mM potassium phosphate buffer, pH 6.3. Specific activity: approximately 8,000 units/mg protein. One unit converts one nanomole of linoleic acid per minute at 20°C. Storage: -80°C.
5000 units

EH-001
Cyclooxygenase (from ram seminal vesicles)
>95%, Supplied in 0.1 M Tris·HC1 containing 1% Tween 80, pH 8.0. Specific activity with arachidonic acid is 24 units/mg protein. One unit will consume one micromole of oxygen per minute at 30°C. Review⁸. Storage: -80°C.
1.0 mg

ANTIBODIES

SA-237
Anti-PGHS-2 (COX-2)
Rabbit polyclonal antibody. Immunogen: Mouse PGHS-2 synthetic peptide conjugated to KLH. Supplied as 1 mg/ml peptide antigen immunoaffinity purified IgG in phosphate buffered saline (PBS). Recognizes MW~72 kDa human, ovine and murine prostaglandin H synthase-2 (cyclooxygenase-2). It does not crossreact with PGHS-1 (Cox-1)⁹. Applications: WB 1/1000, IP, IC.
25 µg
100 µg

REFERENCES

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9. V. Zhang et al. Biochem. Biophys. Res. Commun. 1996 227 499