SH2 AND SH3 DOMAINS

P-320
IRS-1 (Y608) peptide
Lys-Lys-His-Thr-Asp-Asp-Gly-Tyr-Met-Pro-Met-Ser-Pro-Gly-Val-Ala
>97%, MW=1732.8, Storage: -20°C
Sequence is from insulin receptor substrate 1 (IRS-1) inclusive of Tyr-608. It contains the insulin receptor tyrosine kinase substrate motif YMXM (Tyr-Met-X-Met). This peptide has been used as a substrate for purified insulin receptor (K_m=90 µM)¹ and other tyrosine kinases² in phosphocellulose binding assays. The tyrosine phosphorylated version of this peptide binds to phosphatidylinositol 3-kinase SH2 domain and activates the enzyme³.
1 mg
5 x 1 mg

P-301
pp60^c-src C-terminal phosphoregulatory peptide
Thr-Ser-Thr-Glu-Pro-Gln-Tyr(PO₃H₂)-Gln-Pro-Gly-Glu-Asn-Leu
98%, MW=1543.7, Storage: -20°C
This phosphopeptide is the C-terminal negative regulatory domain of c-src (521-533) which includes the negative regulatory site (Tyr-527). This peptide, when tyrosine-phosphorylated, binds to the internal SH2 domain^4,7.
1 mg
5 x 1 mg

P-304
pp60c-src C-terminal peptide
Thr-Ser-Thr-Glu-Pro-Gln-Tyr-Gln-Pro-Gly-Glu-Asn-Leu
>97%, MW=1463.7, Storage: -20°C
Sequence is identical to P-301, above, but it is not phosphorylated.
Useful as a control peptide or as a tyrosine kinase substrate⁴.
1 mg
5 x 1 mg

P-305
hSOS (1149-1158) N10 SH3 binding domain
Val-Pro-Pro-Pro-Val-Pro-Pro-Arg-Arg-Arg
>97%, MW=1169.7, Storage: -20°C
SH3 binding sequence of guanine nucleotide exchange factor hSOS which is a ligand for the adaptor protein Grb2 SH3 domain⁵. Blocks SOS/Grb2 interaction and thereby prevents ras activation via receptor protein tyrosine kinases. K_m=5.6 µM for N-terminal SH3 of Grb2⁶.
1 mg
5 x 1 mg

P-311
PI 3-K SH3 domain binding peptide
Arg-Lys-Leu-Pro-Pro-Arg-Pro-Arg-Arg
>97%, MW=1174.7, Storage: -20°C
Sequence is derived from directed random library using phosphoinositol 3-kinase (PI 3-K) SH3 domain as the ligand. Binds to PI 3-K SH3 tightly (K_m=7.6 µM)⁶.
1 mg
5 x 1 mg

P-300
YEEI peptide
Glu-Pro-Gln-Tyr(PO₃H₂)-Glu-Glu-Ile-Pro-Ile-Tyr-Leu
98%, MW=1473.7, Storage: -20°C
Sequence is from hamster polyoma virus middle T inclusive of Tyr-324⁷. This 11-residue phosphopeptide activates Src family members by binding with high affinity (K_d=3-6 nM for Src SH2) to their SH2 domains^7,8. The crystal structures of this peptide complexed to Src⁹ and Lck¹⁰ SH2 domains have been determined.
1 mg
5 x 1 mg

P-302
Biotinylated YEEI peptide
Biotinyl-w-aminocaproyl-Tyr(PO₃H₂)-Glu-Glu-Ile
98%, MW-972.1, Storage: -20°C
This biotinylated probe binds to SH2 domains of Src family members including Src, Lck, and Fyn⁷.
200 µg
5 x 200 µg


ANTIBODIES

SA-164
Anti-PI3-K, SH2/SH3-containing protein
Mouse monoclonal antibody. Clone CA3


SA-165
Anti-PI3-K, SH2/SH3-containing protein
Mouse monoclonal antibody. Clone AB6.


REFERENCES

1. S.E. Shoelson et al. Proc. Natl. Acad. Sci. USA 1992 89 2027

2. P. Garcia et al. J. Biol. Chem. 1993 268 25146

3. T. Rordorf-Nikolic et al. J. Biol. Chem. 1995 270 3662

4. D.K. Luttrell et al. Proc. Natl. Acad. Sci. USA 1994 91 83

5. N. Li et al. Nature 1993 363 85

6. J.K. Chen et al. J. Am. Chem. Soc. 1993 115 12591

7. Z. Songyang et al. Cell 1993 72 767

8. S.A. Courteneidge et al. J. Virol. 1991 65 3301

9. G. Waksman et al. Cell 1993 72 779

10. M.J. Eck et al. Nature 1993 362 87