In human IVF programmes it is usually sperm, zygotes (preembryos), embryos and recently also blastocysts that are cryopreserved. All freezing methods developed by Medi-Cult rely on the presence of one or more cryoprotectants.
Medi-Cult supplies Embryo Freezing Medium (EFM) and convenient Freezing and Thawing Packs with ready-to-use solutions for a 3 step freezing and thawing procedure.
Cryopreservation Manual -
This standard laboratory cryopreservation reference manual reference is provided free by Nunc.
Association of Tissue Banks - http://www.aatb.org/
Professional organization for accreditation and information.
Seed Storage Laboratory (NSSL) -
The section on "Preservation" discusses cryogenic storage.
LifeNet is the largest, full-service, non-profit allograft Tissue Banking System in the United States. LifeNet is also a non-profit, federally-designated, Organ Procurement Organization providing donation systems for heart, liver, kidney, pancreas,lung, and other organs for transplant.
fruit flies for future research -
An article updating the Peter Mazur research at Oak Ridge National Laboratory. From "Technical Highlights" in the ORNL Review from 1993. You have to page down about halfway for the article on Mazur.
Fruit Fly Embryos Hatched - http://www.ornl.gov/ORNLReview/rev26-1/net1026.html
An article from "Technical Highlights" in the Oak Ridge National Laboratory Review (Vol. 26, No. 1). You have to page down several pages to find this. It reports research of Peter Mazur and others from 1992.
and Cryobiology Research Group -
Cryobiology research at the University of Luton, Bedfordshire, UK, specializing in cell and embryo cryopreservation and freeze-drying.
of Biological Systems - http://www.engr.wisc.edu/groups/mtsm/cryopres/cryo.html
Research on trehalose and cryopreservation at University of Wisconsin, Molecular Thermodynamics and Statistical Mechanics Group.
Application Guide - http://nalgenelab.nalgenunc.com/select/app/cryo/
A manual for the cryopreservation of cells, as a downloadable PDF file (requires Adobe Acrobat plug-in). From Nunc.
Purchases can be made from
TWC BioSearch at any of our offices across China or South East Asia.
Storey's Home Page - http://superior.carleton.ca/~kbstorey/
The "frozen frog" expert at Carleton University, Ottawa, Ontario, Canada
Homepage - http://www-users.rwth-aachen.de/Volker.Rindler/Cryo.html
From Volker Rindler at the Helmholtz-Institute for Biomedical Engineering at the Aachen University of Technology. Emphasis on freeze-drying red blood cells.
Cool Guide to Cryopreservation -
Manual of the general principles of cryobiology, especially regarding the preservation of sperm and embryos.
(journal) - http://www.apnet.com/www/journal/cy.htm
International Journal of Low Temperature Biology and Medicine. Published by the Society for Cryobiology.