TWC BioSearch - ELISA Development Systems

ELISA Development Systems

Matched Antibody Pairs for ELISA R&D Systems offers a comprehensive line of cytokine antibody pairs matched for construction of ELISAs. Each pair has been tested for its ability to sandwich efficiently. The capture antibody is usually a monoclonal antibody and the detection antibody is usually a biotinylated antigen-affinity purified goat polyclonal antibody. We provide a basic protocol for demonstration purpose; you choose the enzyme, substrate, and working concentrations. Please refer to the antibody section for more detailed information on particular antibodies.
Analyte	Use	Catalog #	Size
human ANG (Angiogenin)	capture detection protein	MAB265 BAF265 265-AN-050	500 痢 50 痢 50 痢
human AR (Amphiregulin)	capture detection protein	MAB262 BAF262 262-AR-100	500 痢 50 痢 100 痢
human BDNF	capture detection protein	MAB908 BAF648 248-BD-005	500 痢 50 痢 5 痢
human BTC	capture detection protein	MAB261 BAF261 261-CE-010	500 痢 50 痢 10 痢
human CNTF	capture detection protein	MAB657 BAF257 257-NT-010	500 痢 50 痢 10 痢
rat CNTF	capture detection protein	MAB557 BAF557 557-NT-010	500 痢 50 痢 10 痢
human EGF	capture detection protein	MAB636 BAF236 236-EG-200	500 痢 50 痢 200 痢
human ENA-78	capture detection protein	MAB654 BAF254 254-X-010	500 痢 50 痢 10 痢
human Eotaxin	capture detection protein	MAB320 BAF320 320-EO-020	500 痢 50 痢 20 痢
mouse Eotaxin	capture detection protein	AF-420-NA BAF420 420-ME-020	100 痢 50 痢 20 痢
human Fas	capture detection protein	MAB144 BAF326 326-FS-050	500 痢 50 痢 50 痢
human FGF-4	capture detection protein	MAB635 BAF235 235-F4-025	500 痢 50 痢 25 痢
human FGF-6	capture detection protein	MAB238 BAF238 238-F5-025	500 痢 50 痢 25 痢
human FGF-7	capture detection protein	MAB251 BAF251 251-KG-010	500 痢 50 痢 10 痢
human FGF-9	capture detection protein	MAB273 BAF273 273-F9-025	500 痢 50 痢 25 痢
human Flt-3 Ligand	capture detection protein	MAB608 BAF308 308-FKN-005	500 痢 50 痢 5 痢
human Fractalkine	capture detection protein protein	AF365 BAF365 365-FR-025 362-CX-025	100 痢 50 痢 25 痢 25 痢
human G-CSF	capture detection protein	MAB214 BAF214 214-CS-005	500 痢 50 痢 5 痢
human GDNF	capture detection protein	MAB212 BAF212 212-GD-010	500 痢 50 痢 10 痢
human GM-CSF	capture detection protein	MAB615 BAM215 215-GM-005	500 痢 250 痢 5 痢
mouse GM-CSF	capture detection protein	MAB415 BAF415 415-ML-005	500 痢 50 痢 5 痢
human sgp130	capture detection protein	MAB628 BAF228 228-GP-010	500 痢 50 痢 10 痢
human HGF	capture detection protein	MAB694 BAF294 294-HG-005	500 痢 100 痢 5 痢
human I-309	capture detection protein	MAB272 BAF272 272-I-010	500 痢 50 痢 10 痢
human IFN-g	capture detection protein	MAB285 BAF285 285-IF-100	500 痢 50 痢 100 痢
mouse IFN-g	capture detection protein	MAB785 BAF485 485-MI-100	500 痢 50 痢 100 痢
human IGF-I	capture detection protein	MAB291 BAF291 291-G1-050	500 痢 50 痢 50 痢
human IL-1a	capture detection protein	MAB200 BAF200 200-LA-002	500 痢 50 痢 2 痢
rat IL-1a	capture detection protein	MAB500 BAF500 500-RL-005	500 痢 50 痢 5 痢
human IL-1b	capture detection protein	MAB601 BAF201 201-LB-005	500 痢 50 痢 5 痢
mouse IL-1b	capture detection protein	MAB401 BAF401 401-ML-005	500 痢 50 痢 5 痢
rat IL-1b	capture detection protein	MAB501 BAF501 501-RL-010	500 痢 50 痢 10 痢
human IL-1ra	capture detection protein	MAB280 BAF280 280-RA-010	1 mg 50 痢 10 痢
human IL-1 sRI	capture detection protein	MAB269 BAF269 269-1R-100	500 痢 50 痢 100 痢
human IL-1 sRII	capture detection protein	MAB663 BAF263 263-2R-025	500 痢 50 痢 25 痢
human IL-2	capture detection protein	MAB602 BAF202 202-IL-010	500 痢 50 痢 10 痢
mouse IL-2	capture detection protein	MAB702 BAF402 402-ML-020	500 痢 50 痢 20 痢
human IL-2 Ra	capture detection protein	MAB623 BAF223 223-2A-005	500 痢 50 痢 5 痢
human IL-3	capture detection protein	MAB603 BAF203 203-IL-010	500 痢 50 痢 10 痢
mouse IL-3	capture detection protein	MAB403 BAF403 403-ML-010	500 痢 50 痢 10 痢
human IL-4	capture detection protein	MAB604 BAF204 204-IL-005	500 痢 50 痢 5 痢
mouse IL-4	capture detection protein	MAB404 BAF404 404-ML-005	500 痢 50 痢 5 痢
rat IL-4	capture detection protein	MAB504 BAF504 504-RL-005	500 痢 50 痢 5 痢
mouse IL-5	capture detection protein	MAB405 BAM705 405-ML-005	500 痢 50 痢 5 痢
human IL-6	capture detection protein	MAB206 BAF206 206-IL-010	500 痢 50 痢 10 痢
mouse IL-6	capture detection protein	MAB406 BAF406 406-ML-005	500 痢 50 痢 5 痢
rat IL-6	capture detection protein	MAB506 BAF506 506-RL-010	500 痢 50 痢 10 痢
human IL-6 sR	capture detection protein	MAB227 BAF227 227-SR-025	500 痢 50 痢 25 痢
human IL-7	capture detection protein	MAB207 BAF207 207-IL-005	500 痢 50 痢 5 痢
human IL-8	capture detection protein	MAB208 BAF208 208-IL-010	500 痢 50 痢 10 痢
human IL-10	capture detection protein	MAB217 BAF217 217-IL-005	500 痢 100 痢 5 痢
mouse IL-10	capture detection protein	MAB417 BAF417 417-ML-005	500 痢 50 痢 5 痢
human IL-11	capture detection protein	MAB618 BAF218 218-IL-005	500 痢 50 痢 5 痢
human IL-12 (p40)	capture detection protein	MAB609 BAF219 309-IL-002	500 痢 100 痢 2 痢
human IL-12 (p70)	capture detection protein	MAB611 BAF219 219-IL-005	500 痢 100 痢 5 痢
mouse IL-12 (p70)	capture detection protein	MAB419 BAF419 419-ML-010	500 痢 50 痢 10 痢
human IL-13	capture detection protein	MAB213 BAF213 213-IL-005	500 痢 100 痢 5 痢
mouse IL-13	capture detection protein	MAB413 BAF413 413-ML-005	500 痢 50 痢 5 痢
human IL-15	capture detection protein	MAB647 BAM247 247-IL-005	500 痢 50 痢 5 痢
human IL-16	capture detection protein	AF-316-PB BAF316 316-IL-002	100 痢 50 痢 2 痢
human IL-17	capture detection protein	MAB317 BAF317 317-IL-050	500 痢 50 痢 50 痢
mouse IL-17	capture detection protein	MAB721 BAF421 421-ML-025	500 痢 50 痢 25 痢
human IL-18	capture detection protein	MAB318 BAF318 318-IL-025	500 痢 50 痢 25 痢
mouse IL-18	capture detection protein	MAB422 BAF422 422-ML-025	500 痢 50 痢 25 痢
human IP-10	capture detection protein	MAB266 BAF266 266-IP-010	500 痢 50 痢 10 痢
mouse KC	capture detection protein	MAB453 BAF453 453-KC-010	500 痢 50 痢 10 痢
human Leptin	capture detection protein	MAB398 BAM398 398-MP-01M	500 痢 250 痢 1 mg
mouse/rat Leptin	capture detection protein	AF498 BAF498 498-OB-01M	100 痢 50 痢 1 mg
human LIF	capture detection protein	MAB250 BAF250 250-L-010	500 痢 50 痢 10 痢
human M-CSF	capture detection protein	MAB616 BAF216 216-MC-005	500 痢 50 痢 5 痢
human MCP-1	capture detection protein	MAB679 BAF279 279-MC-010	500 痢 50 痢 10 痢
human MCP-3	capture detection protein	MAB282 BAF282 282-P3-010	500 痢 50 痢 10 痢
human MIF	capture detection protein	MAB289 BAF289 289-MF-002	500 痢 50 痢 2 痢
human MIP-1a	capture detection protein	MAB670 BAF270 270-LD-010	500 痢 50 痢 10 痢
human MIP-1b	capture detection protein	MAB271 BAF271 271-BME-010	500 痢 50 痢 10 痢
human b-NGF	capture detection protein	MAB256 BAF256 256-GF-100	500 痢 50 痢 100 痢
human NT-3	capture detection protein	MAB267 BAF267 267-N3-005	500 痢 50 痢 5 痢
human NT-4	capture detection protein	MAB268 BAF268 268-N4-005	500 痢 50 痢 5 痢
human OSM	capture detection protein	MAB295 BAF295 295-OM-010	500 痢 50 痢 10 痢
human PDGF-BB	capture detection protein	385-PR-100/CF BAF220 220-BB-010	100 痢 50 痢 10 痢
human PlGF	capture detection protein	MAB264 BAF264 264-PG-010	500 痢 50 痢 10 痢
human RANTES	capture detection protein	MAB678 BAF278 278-RN-010	500 痢 50 痢 10 痢
human SCF	capture detection protein	MAB655 BAF255 255-SC-010	500 痢 50 痢 10 痢
mouse SCF	capture detection protein	MAB455 BAF455 455-MC-010	500 痢 50 痢 10 痢
human TARC	capture detection protein	MAB364 BAF364 364-DN-025	500 痢 50 痢 25 痢
TGF-b	capture capture detection protein	341-BR-050 MAB240 BAF240 240-B-002	50 痢 500 痢 50 痢 2 痢
TGF-b	capture detection protein	MAB612 BAF302 302-B2-002	500 痢 50 痢 2 痢
human TGF-b	capture detection protein	MAB243 BAF243 243-B3-002	500 痢 50 痢 2 痢
human TNF-a	capture detection protein	MAB610 BAF210 210-TA-010	500 痢 50 痢 10 痢
mouse TNF-a	capture detection protein	AF-410-NA BAF410 410-MT-010	100 痢 50 痢 10 痢
rat TNF-a	capture detection protein	MAB510 BAF510 510-RT-010	500 痢 50 痢 10 痢
human TNF-b	capture detection protein	MAB611 BAF211 211-TB-010	500 痢 50 痢 10 痢
human TNF RI	capture detection protein	MAB625 BAF225 225-B1-025	500 痢 50 痢 25 痢
human Tpo	capture detection protein	MAB288 BAM288 288-TP-005	500 痢 250 痢 5 痢
mouse Tpo	capture detection protein	MAB488 BAF488 488-TO-005	1 mg 50 痢 5 痢
human VEGF	capture detection protein	AF-293-NA BAF293 293-VE-010	100 痢 50 痢 10 痢

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DuoSet™ ELISA Development Kits DuoSet Kits contain the basic components required for the development of sandwich ELISAs to measure natural and recombinant cytokines. DuoSet components are tested to ensure compatability; however, results between users will vary depending on the coating of the microplates and the assay procedure specifications. Why use a DuoSet instead of a kit? DuoSets are designed to provide researchers experienced in ELISA development with the flexibility to create assays optimized specifically for their needs. ELISA kits are fully developed assay systems and are the best choice for researchers less experienced in ELISA development. Each DuoSet contains: Capture Antibody Standard Detection Antibody Streptavidin-HRP Reagents supplied are sufficient to generate approximately 15 96-well plates.
Analyte	Catalog Number	Pack Size
human IFN-g	DY285	1 Kit
mouse IFN-g	DY485	1 Kit
rat IFN-g	80-4737-00	1 Kit
mouse IL-1b	DY401	1 Kit
human IL-2	DY202	1 Kit
mouse IL-2	DY402	1 Kit
human IL-4	DY204	1 Kit
mouse IL-4	DY404	1 Kit
human IL-5	80-5025-00	1 Kit
human IL-6	DY206	1 Kit
human IL-8	DY208	1 Kit
mouse IL-10	80-4863-00	1 Kit
human IL-12 p40	DY1240	1 Kit
mouse IL-12 p40	DY499	1 Kit
mouse IL-12 p70	DY419	1 Kit
human TGF-b1	DY240	1 Kit
human TNF-a1	DY210	1 Kit
mouse TNF-a1	DY410	1 Kit

THESE PRODUCTS ARE FOR RESEARCH USE ONLY.
NOT FOR USE IN DIAGNOSTIC PROCEDURES.

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