The columns operate on a negative selection principle, with monocytes, B cells, NK cells and granulocytes preferentially targeted for removal. Enriched T cells pass through the column and are recovered in the sterile effluent ready for tissue culture. Enrichment of cells via a negative cell selection strategy minimizes any potential activation of cells associated with positive selection techniques.
T cell columns have a wide range of loading capacities and are available in several sizes for processing variable numbers of cells. Columns enrich for either all CD3+ cells, or for subsets of T cells, such as: CD4+, CD8+, CD4+/CD45RA-, or CD4+/CD45RO- cells. T cell subset columns are supplied with an antibody cocktail designed to negatively deplete the cell population of no interest, thereby enriching for the desired T cell subset.
|
Column |
Size |
Catalog # |
# of Columns |
Column Capacity |
|
human CD3+ |
mini |
10 |
up to 100 x 106 |
|
|
25 |
up to 100 x 106 |
|||
|
small |
5 |
50-300 x 106 |
||
|
10 |
50-300 x 106 |
|||
|
25 |
50-300 x 106 |
|||
|
large |
1 |
300-1000 x 106 |
||
|
mouse CD3+ |
mini |
10 |
up to 100 x 106 |
|
|
25 |
up to 100 x 106 |
|||
|
small |
5 |
50-300 x 106 |
||
|
10 |
50-300 x 106 |
|||
|
25 |
50-300 x 106 |
|||
|
large |
1 |
300-1000 x 106 |
||
|
rat CD3+ |
small |
5 |
50-300 x 106 |
|
|
10 |
50-300 x 106 |
|||
|
25 |
50-300 x 106 |
|||
|
human CD4+ |
mini |
4 |
up to 100 x 106 |
|
|
standard |
4 |
up to 200 x 106 |
||
|
mouse CD4+ |
mini |
4 |
up to 100 x 106 |
|
|
standard |
4 |
up to 200 x 106 |
||
|
human CD4+/45RA- |
standard |
4 |
up to 200 x 106 |
|
|
human CD4+/45RO- |
standard |
4 |
up to 200 x 106 |
|
|
human CD8+ |
standard |
4 |
up to 200 x 106 |
|
|
mouse CD8+ |
standard |
4 |
up to 200 x 106 |
This page is